Bordetella pertussisLipopolysaccharide Resists the Bactericidal Effects of Pulmonary Surfactant Protein A

Surfactant Protein A Resists the Bactericidal Effects of Pulmonary Lipopolysaccharide Bordetella pertussis

Effect of pulmonary surfactant protein

The effects of surfactant-associated protein A (SPA) on lipid adsorption to the air-water interface and accumulation of dipalmitoylphosphatidylcholine (DPPC) in the surface region were investigated at 37%. Dispersions used were bovine pulmonary lipid extract surfactant with or without neutral lipid (NL). Lipid adsorption was examined with the Wilhelmy plate technique and DPPC accumulation by mo...

Disparate effects of two phosphatidylcholine binding proteins, C-reactive protein and surfactant protein A, on pulmonary surfactant structure and function.

C-reactive protein (CRP) and surfactant protein A (SP-A) are phosphatidylcholine (PC) binding proteins that function in the innate host defense system. We examined the effects of CRP and SP-A on the surface activity of bovine lipid extract surfactant (BLES), a clinically applied modified natural surfactant. CRP inhibited BLES adsorption to form a surface-active film and the film's ability to lo...

Pulmonary surfactant protein A interacts with gel-like regions in monolayers of pulmonary surfactant lipid extract.

Epifluorescence microscopy was used to investigate the interaction of pulmonary surfactant protein A (SP-A) with spread monolayers of porcine surfactant lipid extract (PSLE) containing 1 mol % fluorescent probe (NBD-PC) spread on a saline subphase (145 mM NaCl, 5 mM Tris-HCl, pH 6.9) containing 0, 0.13, or 0.16 microg/ml SP-A and 0, 1.64, or 5 mM CaCl(2). In the absence of SP-A, no differences ...

Surfactant protein profile of pulmonary surfactant in premature infants.

Although premature infants are known to be deficient in pulmonary surfactant, there is limited information regarding surfactant protein (SP) composition. To assess the postnatal profile of SPs, tracheal aspirate samples were collected from 35 intubated infants of 23-31 weeks of gestation between 8 and 80 days of age. In 71 large aggregate surfactant samples that had normal in vitro function (mi...